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A heterotrophic nitrification-aerobic denitrification bacterium TJPU05 suitable for nitrogen removal

《环境科学与工程前沿（英文）》 2022年 第16卷 第6期 doi: 10.1007/s11783-021-1503-6

摘要：

• H. venusta TJPU05 showed excellent HN-AD ability at high salinity.

关键词： Salt-tolerant bacteria     H. venusta TJPU05     Heterotrophic nitrification and aerobic denitrification     High-salinity wastewater    

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Characterization of CANON reactor performance and microbial community shifts with elevated COD/N ratios under a continuous aeration mode

Yao Zhang, Yayi Wang, Yuan Yan, Haicheng Han, Min Wu

《环境科学与工程前沿（英文）》 2019年 第13卷 第1期 doi: 10.1007/s11783-019-1095-6

摘要：

COD/N at low ratios (0–0.82) improved N removals of CANON.

CANON performance decreased after COD/N up to 0.82.

The relative abundance of AOB decreased continuously with increasing COD/N.

AOB outcompeted at a high COD load led to CANON failure.

The relative abundance of AnAOB decreased and increased with increasing COD/N.

关键词： CANON process     COD/N ratio     Anammox     Ammonia oxidizing bacteria     Aerobic heterotrophic bacteria    

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Nitrifying and denitrifying bacteria in aerobic granules formed in sequencing batch airlift reactors

WANG Fang, YANG Fenglin, QI Aijiu

《环境科学与工程前沿（英文）》 2007年 第1卷 第2期   页码 184-189 doi: 10.1007/s11783-007-0032-2

摘要： The purpose of this study was to investigate nitrifying bacteria and denitrifying bacteria isolated from aerobic granules. Aerobic granules were formed in an internal-circulate sequencing batch airlift reactor (SBAR) and biodegradation of NH3--N was analyzed in the reactor. Bacteria were isolated and determined from aerobic granules using selected media. The growth properties and morphology of bacteria colonies were observed by controlling aerobic or anaerobic conditions in the culture medium. It was found that bacteria in aerobic granules were diverse and some of them were facultative aerobes. The diversity of bacteria in aerobic granules was a premise of simultaneous nitrification and denitrification.
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Micro-analysis of nitrogen transport and conversion inside activated sludge flocs using microelectrodes

Lei WANG, Yongtao LV, Xudong WANG, Yongzhe YANG, Xiaorong BAI

《环境科学与工程前沿（英文）》 2011年 第5卷 第4期   页码 633-638 doi: 10.1007/s11783-011-0329-z

摘要： To investigate the nitrogen transport and conversion inside activated sludge flocs, micro-profiles of O , NH , NO , NO , and pH were measured under different operating conditions. The flocs were obtained from a laboratory-scale sequencing batch reactor. Nitrification, as observed from interfacial ammonium and nitrate fluxes, was higher at pH 8.5, than at pH 6.5 and 7.5. At pH 8.5, heterotrophic bacteria used less oxygen than nitrifying bacteria, whereas at lower pH heterotrophic activity dominated. When the ratio of C to N was decreased from 20 to 10, the ammonium uptake increased. When dissolved oxygen (DO) concentration in the bulk liquid was decreased from 4 to 2 mg·L , nitrification decreased, and only 25% of the DO influx into the flocs was used for nitrification. This study indicated that nitrifying bacteria became more competitive at a higher DO concentration, a higher pH value (approximately 8.5) and a lower C/N.

关键词： nitrogen transport     activated sludge flocs     heterotrophic bacteria     nitrifying bacteria     microelectrodes    

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functionality and network differentiation of activated sludge community in a full-scale anaerobic/anoxic/aerobic

《环境科学与工程前沿（英文）》 2023年 第17卷 第3期 doi: 10.1007/s11783-023-1636-x

摘要：

● Environmental parameters affected functional bacteria and network associations.

关键词： Activated sludge     Bacterial community     Tank-dependence     Network association     Functional bacteria    

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Microprofiles of activated sludge aggregates using microelectrodes in completely autotrophic nitrogen removal over nitrite (CANON) reactor

Yongtao LV,Xuan CHEN,Lei WANG,Kai JU,Xiaoqiang CHEN,Rui MIAO,Xudong WANG

《环境科学与工程前沿（英文）》 2016年 第10卷 第2期   页码 390-398 doi: 10.1007/s11783-015-0818-6

摘要： Microsensor measurements and fluorescence in situ hybridization (FISH) analysis were combined to investigate the microbial populations and activities in a laboratory-scale sequencing batch reactor (SBR) for completely autotrophic nitrogen removal over nitrite (CANON). Fed with synthetic wastewater rich in ammonia, the SBR removed 82.5±5.4% of influent nitrogen and a maximum nitrogen-removal rate of 0.52 kgN·m ·d was achieved. The FISH analysis revealed that aerobic ammonium-oxidizing bacteria (AerAOB) and anaerobic ammonium-oxidizing bacteria (AnAOB) dominated the community. To quantify the microbial activities inside the sludge aggregates, microprofiles were measured using pH, dissolved oxygen (DO), , and microelectrodes. In the outer layer of sludge aggregates (0–700 μm), nitrite-oxidizing bacteria (NOB) showed high activity with 4.1 μmol·cm ·h of maximum nitrate production rate under the condition of DO concentration higher than 3.3 mg·L . Maximum AerAOB activity was detected in the middle layer (depths around 1700 μm) where DO concentration was 1.1 mg·L . In the inner layer (2200–3500 μm), where DO concentration was below 0.9 mg·L , AnAOB activity was detected. We thus showed that information obtained from microscopic views can be helpful in optimizing the SBR performance.

关键词： microelectrodes     CANON     aerobic ammonium-oxidizing bacteria     anaerobic ammonium-oxidizing bacteria     nitrite-oxidizing bacteria    

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Cultivation of aerobic granular sludge in a conventional, continuous flow, completely mixed activated

Xi CHEN,Linjiang YUAN,Wenjuan LU,Yuyou LI,Pei LIU,Kun NIE

《环境科学与工程前沿（英文）》 2015年 第9卷 第2期   页码 324-333 doi: 10.1007/s11783-014-0627-3

摘要： Aerobic granules were formed in a conventional, continuous flow, completely mixed activated sludge system (CMAS). The reactor was inoculated with seed sludge containing few filaments and fed with synthetic municipal wastewater. The settling time of the sludge and the average dissolved oxygen (DO) of the reactor were 2 h and 4.2 mg·L , respectively. The reactor was agitated by a stirrer, with a speed of 250 r·min , to ensure good mixing．The granular sludge had good settleability, and the sludge volume index (SVI) was between 50 and 90 mL·g . The laser particle analyzer showed the diameter of the granules to be between 0.18 and 1.25 mm. A scanning electron microscope (SEM) investigation revealed the predominance of sphere-like and rod-like bacteria, and only few filaments grew in the granules. The microbial community structure of the granules was also analyzed by polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE). Sequencing analysis indicated the dominant species were α, β, and γ- , , and . The data from the study suggested that aerobic granules could form, if provided with sufficient number of filaments and high shear force. It was also observed that a high height-to-diameter ratio of the reactor and short settling time were not essential for the formation of aerobic granular sludge.

关键词： aerobic granular sludge     completely mixed activated sludge system (CMAS)     continuous flow     shear force     filamentous bacteria     polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE)    

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Impact of total organic carbon and chlorine to ammonia ratio on nitrification in a bench-scale drinking water distribution system

Yongji ZHANG, Lingling ZHOU, Guo ZENG, Huiping DENG, Guibai LI

《环境科学与工程前沿（英文）》 2010年 第4卷 第4期   页码 430-437 doi: 10.1007/s11783-010-0247-5

摘要： Nitrification occurs in chloraminated drinking water systems and is affected by water quality parameters. The aim of this study was to investigate the impact of total organic carbon and chlorine to ammonia ratio on nitrification potential in a simulated drinking water distribution system as during chloramination. The occurrence of nitrification and activity of nitrifying bacteria was primarily monitored using four rotating annular bioreactors (RAB) with different chlorine to ammonia ratios and total organic carbon (TOC) levels. The results indicated that nitrification occurred despite at a low influent concentration of ammonia, and a high concentration of nitrite nitrogen was detected in the effluent. The study illustrated that reactors 1(R1) and 3 (R3), with higher TOC levels, produced more nitrite nitrogen, which was consistent with the ammonia-oxidizing bacteria (AOB) counts, and was linked to a relatively more rapid decay of chloramines in comparison to their counterparts (R2 and R4). The AOB and HPC counts were correlated during the biofilm formation with the establishment of nitrification. Biofilm AOB abundance was also higher in the high TOC reactors compared with the low TOC reactors. The chlorine to ammonia ratio did not have a significant impact on the occurrence of nitrification. Bulk water with a high TOC level supported the occurrence of nitrification, and AOB development occurred at all examined chlorine to ammonia dose ratios (3∶1 or 5∶1).

关键词： nitrification     drinking water     ammonia- oxidizing bacteria (AOB)     chloramines     organic carbon     heterotrophic bacteria    

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Toxic effect of sodium perfluorononyloxy-benzenesulfonate on in aerobic denitrification, cell structure

《环境科学与工程前沿（英文）》 2021年 第15卷 第5期 doi: 10.1007/s11783-021-1391-9

摘要：

• OBS inhibited the growth of P. stutzeri and destroyed its structure.

关键词： Sodium perfluorononyloxy-benzenesulfonate     Aerobic denitrification     Pseudomonas stutzeri     Ecotoxicity     ROS     Persist organic pollutants     Toxicity     Denitrification     Microbiology    

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Optimization of thermophilic anaerobic-aerobic treatment system for Palm Oil Mill Effluent (POME)

Yijing CHAN,Meifong CHONG,Chunglim LAW

《环境科学与工程前沿（英文）》 2015年 第9卷 第2期   页码 334-351 doi: 10.1007/s11783-014-0626-4

摘要： Optimization of an integrated anaerobic-aerobic bioreactor (IAAB) treatment system for the reduction of organic matter (Chemical Oxygen Demand (COD), Biochemical Oxygen Demand (BOD) and Total Suspended Solids (TSS) concentrations) in Palm Oil Mill Effluent (POME) to legal standards with high methane yield was performed for the first time under thermophilic condition (50°C–55°C) by using response surface methodology (RSM). The experiments were conducted based on a central composite rotatable design (CCRD) with three independent operating variables, organic loading rates in anaerobic compartment (OLR ) and mixed liquor volatile suspended solids (MLVSS) concentration in anaerobic (MLVSS ) and aerobic compartments (MLVSS ). The optimum conditions for the POME treatment were determined as OLR of 15.6 g COD·L ·d , MLVSS of 43100 mg·L , and MLVSS of 18600 mg·L , where high aerobic COD, BOD and TSS removal efficiencies of 96.3%, 97.9%, and 98.5% were achieved with treated BOD of 56 mg·L and TSS of 28 mg·L meeting the discharge standard. This optimization study successfully achieved a reduction of 42% in the BOD concentrations of the final treated effluent at a 48% higher OLR as compared to the previous works. Besides, thermophilic IAAB system scores better feasibility and higher effectiveness as compared to the optimized mesophilic system. This is due to its higher ability to handle high OLR with higher overall treatment efficiencies (more than 99.6%), methane yield (0.31 L CH ·g COD ) and purity of methane (67.5%). Hence, these advantages ascertain the applicability of thermophilic IAAB in the POME treatment or even in other high-strength wastewaters treatment.

关键词： palm oil mill effluent (POME)     anaerobic     aerobic     thermophilic     biogas     optimization    

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The size distribution of airborne bacteria and human pathogenic bacteria in a commercial composting plant

《环境科学与工程前沿（英文）》 2021年 第15卷 第3期 doi: 10.1007/s11783-020-1356-4

摘要：

•Bacterial concentrations from eight stages were 104–105copies/m3.

关键词： Size distribution     Airborne bacteria     Biological diversity     Human pathogenic bacteria     Composting plants    

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Characteristics of night soil and leaf co-composting using aerobic static method

Xiaojie SUN, Dunqiu WANG, Wenjing LU, Hongtao WANG

《环境科学与工程前沿（英文）》 2012年 第6卷 第3期   页码 421-427 doi: 10.1007/s11783-011-0380-9

摘要： The main purpose of this work is to investigate the characteristics of night soil and leaf co-composting using aerobic static composting method. Three influencing factors, including proportion of night soil and leaf, aeration rate and aeration pattern, were investigated through the evolution of the principal physicochemical properties, i.e., temperature, oxygen consumption rate, organic matters, moisture content, carbon, nitrogen, carbon-to-nitrogen ratio and Germination index (GI). It was found that the 3∶1 (w∶w) mixture of night soil and leaf was capable of achieving the highest composting temperature, longest retention time of high temperature (55°C), and fastest organic matter degradation. The 0.14 m ·min ·m aeration rate was most beneficial to composting, and the mixture of night soil and leaf maintained the highest temperature for the longest duration and achieved the highest CO content and GI. The continuous aeration pattern during composting was superior to an intermittent aeration pattern, since the latter delayed the composting process.

关键词： night soil     leaf     aerobic compost     aeration rate     aeration pattern    

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Electroactivity of the magnetotactic bacteria AMB-1 and MSR-1

《环境科学与工程前沿（英文）》 2023年 第18卷 第4期 doi: 10.1007/s11783-024-1808-3

摘要：

● The first study of electrochemically active magnetotactic bacteria.

关键词： Magnetotactic bacteria     Magnetospirillum magneticum     Magnetospirillum gryphiswaldense     Extracellular electron transfer     Microbial fuel cells    

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Aerobic granulation of pure bacterial strain

ADAV Sunil S., LEE Duu-Jong

《环境科学与工程前沿（英文）》 2008年 第2卷 第4期   页码 461-467 doi: 10.1007/s11783-008-0066-0

摘要： The objective of this study is to cultivate aerobic granules by pure bacterial strain, , in a sequencing batch reactor. Stable granules sized 2.0–2.2 mm were formed in the reactor after a five-week cultivation. These granules exhibited excellent settling attributes, and degraded phenol at rates of 1.49 and 1.19 g phenol/(g VSS·d) at 250 and 1500 mg/L of phenol concentration, respectively. Confocal laser scanning microscopic test results show that was distributed over the initial small aggregates, and the outer edge of the granule was away from the core regime in the following stage.

关键词： following     excellent settling     phenol/     sequencing     microscopic    

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Environmental effects and risk control of antibiotic resistance genes in the organic solid waste aerobic

《环境科学与工程前沿（英文）》 2021年 第15卷 第6期 doi: 10.1007/s11783-021-1415-5

摘要：

• ARGs were detected in livestock manure, sludge, food waste and fermentation dregs.

关键词： Antibiotic resistance genes     Organic solid waste     Aerobic composting     Livestock manure     Sludge    

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标题 作者 时间 类型 操作

A heterotrophic nitrification-aerobic denitrification bacterium TJPU05 suitable for nitrogen removal

期刊论文

Characterization of CANON reactor performance and microbial community shifts with elevated COD/N ratios under a continuous aeration mode

Yao Zhang, Yayi Wang, Yuan Yan, Haicheng Han, Min Wu

期刊论文

Nitrifying and denitrifying bacteria in aerobic granules formed in sequencing batch airlift reactors

WANG Fang, YANG Fenglin, QI Aijiu

期刊论文

Micro-analysis of nitrogen transport and conversion inside activated sludge flocs using microelectrodes

Lei WANG, Yongtao LV, Xudong WANG, Yongzhe YANG, Xiaorong BAI

期刊论文

functionality and network differentiation of activated sludge community in a full-scale anaerobic/anoxic/aerobic

期刊论文

Microprofiles of activated sludge aggregates using microelectrodes in completely autotrophic nitrogen removal over nitrite (CANON) reactor

Yongtao LV,Xuan CHEN,Lei WANG,Kai JU,Xiaoqiang CHEN,Rui MIAO,Xudong WANG

期刊论文

Cultivation of aerobic granular sludge in a conventional, continuous flow, completely mixed activated

Xi CHEN,Linjiang YUAN,Wenjuan LU,Yuyou LI,Pei LIU,Kun NIE

期刊论文

Impact of total organic carbon and chlorine to ammonia ratio on nitrification in a bench-scale drinking water distribution system

Yongji ZHANG, Lingling ZHOU, Guo ZENG, Huiping DENG, Guibai LI

期刊论文

Toxic effect of sodium perfluorononyloxy-benzenesulfonate on in aerobic denitrification, cell structure

期刊论文

Optimization of thermophilic anaerobic-aerobic treatment system for Palm Oil Mill Effluent (POME)

Yijing CHAN,Meifong CHONG,Chunglim LAW

期刊论文

The size distribution of airborne bacteria and human pathogenic bacteria in a commercial composting plant

期刊论文

Characteristics of night soil and leaf co-composting using aerobic static method

Xiaojie SUN, Dunqiu WANG, Wenjing LU, Hongtao WANG

期刊论文

Electroactivity of the magnetotactic bacteria AMB-1 and MSR-1

期刊论文

Aerobic granulation of pure bacterial strain

ADAV Sunil S., LEE Duu-Jong

期刊论文

Environmental effects and risk control of antibiotic resistance genes in the organic solid waste aerobic

期刊论文